从表达人衰变加速因子（DAF）的鼠类包装细胞制备的逆转录病毒获得了对人血清的抗性。

Retroviruses prepared from human DAF expressing murine packaging cells acquire resistance against human serum.

作者信息

Hiasa A, Watanabe M, Okada H, Ikenaka K, Fujita T, Yoshimatsu T, Kanematsu T, Shiku H

机构信息

Second Department of Internal Medicine, Mie University School of Medicine, Tsu, Mie 514-8507, Japan.

出版信息

Int J Oncol. 1999 Jun;14(6):1091-6. doi: 10.3892/ijo.14.6.1091.

DOI:10.3892/ijo.14.6.1091

PMID:10339663

Abstract

The complement system of the human body inactivates the infectious ability of retroviruses injected as an artificial gene transfer vector. We established new murine leukemia virus (MuLV) packaging cell lines; D2SS and D7S which express decay-accelerating factor (DAF) on their surface. Both D2SS and D7S were resistant against incubation with fresh human serum. Moreover, the retroviruses produced from these packaging cell lines were also resistant to serum treatment. This resistance can be inhibited by DAF neutralizing antibody 1C6. These data demostrate that DAF induces a partial protection of MuLV infection from the human complement system.

摘要

人体的补体系统可使作为人工基因转移载体注入的逆转录病毒的感染能力失活。我们建立了新的小鼠白血病病毒（MuLV）包装细胞系；D2SS和D7S，它们在其表面表达衰变加速因子（DAF）。D2SS和D7S都对与新鲜人血清孵育具有抗性。此外，从这些包装细胞系产生的逆转录病毒也对血清处理具有抗性。这种抗性可被DAF中和抗体1C6抑制。这些数据表明，DAF可诱导对MuLV感染的部分保护，使其免受人类补体系统的影响。