Alfei L, Aita M, Caronti B, De Vita R, Margotta V, Medolago Albani L, Valente A M
Department of Animal and Human Biology, University of Rome La Sapienza, Italy.
Eur J Histochem. 1999;43(1):29-38.
The role of the hyaluronate receptor, CD44, is well known in adult mammal astrocytes where it modulates neuron-glia interactions. However, no data exist regarding its expression in other vertebrates during their development. In order to detect the expression of CD44 in the chicken and its possible involvement in glial precursor migratory patterns during spinal cord development, a monoclonal antibody (MoAb) against the mammalian standard isoform, CD44-H, was used in immunohistochemical and immunoblot assays. With these methods, CD44 hyaluronate receptors were found on mature astrocyte membranes of adult chicken spinal cord. Astrocytes were identified using a MoAb against GFAP. During development, small clusters of CD44 labelled cells were seen lining the central canal starting from embryonic stage E10. These labelled cells were dispersed in the dorsal, lateral and ventral funiculi of the spinal cord in the subsequent stages. After stage E15, the CD44 labelled cells were identified as astrocytes because of their GFAP immunoreactivity. We conclude that CD44 receptors on immature astrocyte precursors should be considered as early astrocyte markers which have a possible role during cell migratory dispersal.
透明质酸盐受体CD44在成年哺乳动物星形胶质细胞中的作用已为人熟知，它在其中调节神经元与神经胶质的相互作用。然而，关于其在其他脊椎动物发育过程中的表达情况尚无数据。为了检测CD44在鸡中的表达及其在脊髓发育过程中可能参与神经胶质前体细胞迁移模式的情况，一种针对哺乳动物标准异构体CD44-H的单克隆抗体（MoAb）被用于免疫组织化学和免疫印迹分析。通过这些方法，在成年鸡脊髓的成熟星形胶质细胞膜上发现了CD44透明质酸盐受体。使用针对GFAP的MoAb来鉴定星形胶质细胞。在发育过程中，从胚胎期E10开始，可见小群CD4标记的细胞排列在中央管周围。在随后的阶段，这些标记细胞分散在脊髓的背侧、外侧和腹侧索中。在E15期之后，由于其GFAP免疫反应性，CD44标记的细胞被鉴定为星形胶质细胞。我们得出结论，未成熟星形胶质前体细胞上的CD44受体应被视为早期星形胶质细胞标记物，它们在细胞迁移扩散过程中可能发挥作用。
