Pineau P, Nagai H, Prigent S, Wei Y, Gyapay G, Weissenbach J, Tiollais P, Buendia M A, Dejean A
Unité de Recombinaison & Expression Génétique, INSERM U163, Institut Pasteur, Paris, France.
Oncogene. 1999 May 20;18(20):3127-34. doi: 10.1038/sj.onc.1202648.
The chromosome 8p is associated with a large number of allelic imbalances in epithelial tumors including hepatocellular carcinoma (HCC). However, no tumor suppressor gene has been identified so far in this particular region of the genome. To further clarify the pattern of allelic deletions on chromosome 8p in HCC, we have undertaken high-density polymorphic marker analysis of 109 paired normal and primary tumor samples using 40 microsatellites positioned every 2 cm in average throughout 8p. We found that 60% of the tumors exhibited loss of heterozygosity (LOH) at one or more loci at 8p with three distinct minimal deleted areas: a 13 cm region in the distal part of 8p21, a 9 cm area in the more proximal portion of 8p22 and a 5 cm area in 8p23. These data strongly suggest the presence of at least three novel tumor suppressor loci on 8p in hepatocellular carcinoma.
8号染色体短臂（8p）与包括肝细胞癌（HCC）在内的多种上皮肿瘤中的大量等位基因失衡有关。然而，迄今为止在基因组的这一特定区域尚未鉴定出肿瘤抑制基因。为了进一步阐明HCC中8号染色体短臂上等位基因缺失的模式，我们使用平均每隔2厘米定位在整个8p上的40个微卫星，对109对正常和原发性肿瘤样本进行了高密度多态性标记分析。我们发现，60%的肿瘤在8p的一个或多个位点表现出杂合性缺失（LOH），有三个不同的最小缺失区域：8p21远端的一个13厘米区域、8p22近端部分的一个9厘米区域和8p23的一个5厘米区域。这些数据有力地表明，肝细胞癌中8p上至少存在三个新的肿瘤抑制基因座。
