Uccelletti D, Farina F, Palleschi C
Pasteur Institute-Cenci Bolognetti Foundation, Department of Cell and Developmental Biology, University of Rome La Sapienza, Italy.
Yeast. 1999 May;15(7):593-9. doi: 10.1002/(SICI)1097-0061(199905)15:7<593::AID-YEA405>3.0.CO;2-2.
A novel P-type Ca(2+)-ATPase gene has been cloned and sequenced in the yeast Kluyveromyces lactis. The gene has been named KlPMR1 and is localized on chromosome I. The putative gene product contains 936 residues and has a calculated molecular weight of 102,437 Da. Analysis of deduced amino acid sequence (KlPmr1p) indicated that the encoded protein retains all the highly conserved domains characterizing the P-type ATPases. KlPmr1p shares 71% amino acid identity with Pmr1p of S. cerevisiae, 62% with HpPmr1p of Hansenula polymorpha, 56% with Y1Pmr1p of Yarrowia lipolytica and 52% with the Ca(2+)-ATPase encoded for by the SPCA1 gene of Rattus norvegicus; these similarities place KlPmr1p in the SPCA group (secretory pathway Ca(2+)-ATPase) of the P-type ATPases. The K. lactis strain harbouring the Klpmr1 disrupted gene is not able to grow in presence of low calcium concentrations and shows hypersensitivity to high concentrations of EGTA in the medium. These defects are relieved by PMR1 of S. cerevisiae on a centromeric plasmid, demonstrating that KlPMR1 encodes for a functional Pmr1p homologue.
在乳酸克鲁维酵母中克隆并测序了一个新的P型Ca(2 +)-ATP酶基因。该基因被命名为KlPMR1，定位于I号染色体上。推测的基因产物含有936个残基，计算分子量为102,437 Da。对推导的氨基酸序列（KlPmr1p）的分析表明，编码的蛋白质保留了所有表征P型ATP酶的高度保守结构域。KlPmr1p与酿酒酵母的Pmr1p氨基酸同一性为71%，与多形汉逊酵母的HpPmr1p为62%，与解脂耶氏酵母的Y1Pmr1p为56%，与褐家鼠SPCA1基因编码的Ca(2 +)-ATP酶为52%；这些相似性将KlPmr1p置于P型ATP酶的SPCA组（分泌途径Ca(2 +)-ATP酶）中。携带Klpmr1破坏基因的乳酸克鲁维酵母菌株在低钙浓度下不能生长，并且对培养基中高浓度的EGTA表现出超敏感性。酿酒酵母的PMR1在着丝粒质粒上可缓解这些缺陷，表明KlPMR1编码一种功能性的Pmr1p同源物。
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