乳酸克鲁维酵母磷酸烯醇式丙酮酸羧激酶编码基因的分离与核苷酸序列分析
Isolation and nucleotide sequence of the gene encoding phosphoenolpyruvate carboxykinase from Kluyveromyces lactis.
作者信息
Kitamoto H K, Ohmomo S, Iimura Y
机构信息
National Institute of Agrobiological Resources, Ibaraki, Japan.
出版信息
Yeast. 1998 Jul;14(10):963-7. doi: 10.1002/(SICI)1097-0061(199807)14:10<963::AID-YEA282>3.0.CO;2-X.
The KlPCK1 gene encoding phosphoenolpyruvate carboxykinase (PEPCK; ATP-dependent) was cloned from the Kluyveromyces lactis genome using a PCR amplicon from Saccharomyces cerevisiae PCK1 gene as a probe. A DNA fragment of about 4.8 kb containing KlPCK1 complemented PEPCK activity of the mutant of S. cerevisiae defective in PEPCK. The KlPCK1 gene has an open reading frame of 1629 bp (543 amino acids). The KlPCK1 nucleotide sequence and deduced amino acid sequence showed 76% and 84% homologies to those of S. cerevisiae PCK1, respectively. Multiple alignment of ATP-dependent PEPCK genes shows highly conserved regions.
使用来自酿酒酵母PCK1基因的PCR扩增子作为探针，从乳酸克鲁维酵母基因组中克隆了编码磷酸烯醇式丙酮酸羧激酶（PEPCK；ATP依赖性）的KlPCK1基因。一个包含KlPCK1的约4.8 kb DNA片段补充了PEPCK缺陷的酿酒酵母突变体的PEPCK活性。KlPCK1基因有一个1629 bp的开放阅读框（543个氨基酸）。KlPCK1核苷酸序列和推导的氨基酸序列与酿酒酵母PCK1的序列分别显示出76%和84%的同源性。ATP依赖性PEPCK基因的多序列比对显示出高度保守的区域。
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