Berdina N A, Rodionov I M
Pflugers Arch. 1976 Nov 30;367(1):37-42. doi: 10.1007/BF00583654.
Phosphorylase activity was found to increase 2-fold as compared with the control value (79% and 39% respectively) within the first 2-3 s of the vasomotor effect elicited in the skeletal muscle by sympathetic stimulation. Phosphorylase activity was still high during the period of maximum blood flow, although to a lesser extent (54% and 45%). During the phase of restoration of the initial rate of blood flow the values of phosphorylase activity were the same in the experimental and the control sample. The AMP content did not increase with the initiation of the vasomotor effect, and this fact suggests that the increase in phosphorylase activity is due to an increase in phosphorylase "a" rather than to the activation of phosphorylase "b" by AMP. Atropine blocks both the dilator effect and phosphorylase activation elicited by sympathetic stimulation. Acetylcholine, injected intraarterially or added to a muscle homogenate, increases phosphorylase activity. It is concluded that acetylcholine, released by sympathetic nerve endings, performs a second mediator function, that of activating anaerobic metabolism in the skeletal muscle. The two mediator functions of acetylcholine seem to be spatially delimited.
与对照值相比，在交感神经刺激引起骨骼肌血管运动效应的最初2 - 3秒内，磷酸化酶活性增加了2倍（分别为79%和39%）。在最大血流量期间，磷酸化酶活性仍然很高，尽管程度较小（54%和45%）。在血流初始速率恢复阶段，实验样本和对照样本中磷酸化酶活性值相同。血管运动效应开始时，AMP含量并未增加，这一事实表明磷酸化酶活性的增加是由于磷酸化酶“a”的增加，而非AMP对磷酸化酶“b”的激活。阿托品可阻断交感神经刺激引起的舒张效应和磷酸化酶激活。动脉内注射或添加到肌肉匀浆中的乙酰胆碱可增加磷酸化酶活性。得出的结论是，交感神经末梢释放的乙酰胆碱具有第二种介质功能，即激活骨骼肌中的无氧代谢。乙酰胆碱的这两种介质功能似乎在空间上是有界限的。
