Vorob'ev A A, Ukraintsev A D, Berezhnoĭ A M, Zemskov E M
Zh Mikrobiol Epidemiol Immunobiol. 1976 Oct(10):98-102.
Experiments were conducted in vitro. A study was made of the action of the enzymes and of the secretions of the gastrointestinal tract on the viability of the EB vaccine strain. Sensitivity of bacterial cells to the action of saliva, gastric juice, intestinal contents, trypsin, and bile proved to differ. Gastric juice inactivated the vaccine strain which expressed a satisfactory resistance towards the rest of the factors under study practically immediately. There were revealed no significant morphological changes in the cells subjected to the two-hour action of saliva, bile, trypsin and the intestinal content, by means of the electron microscopic examination. These studies permitted to substantiate the rational form of the preparation for oral administration.
实验在体外进行。研究了酶以及胃肠道分泌物对EB疫苗株活力的作用。结果表明，细菌细胞对唾液、胃液、肠内容物、胰蛋白酶和胆汁作用的敏感性存在差异。胃液使疫苗株失活，而疫苗株对其他所研究的因素几乎立即表现出令人满意的抗性。通过电子显微镜检查发现，经唾液、胆汁、胰蛋白酶和肠内容物两小时作用的细胞没有明显的形态变化。这些研究为口服制剂的合理剂型提供了依据。
