Hornok S, Széll Z, Shibalova T A, Varga I
Department of Parasitology and Zoology, University of Veterinary Science, Budapest, Hungary.
Acta Vet Hung. 1999;47(2):207-16. doi: 10.1556/004.47.1999.2.6.
The effects exerted by human recombinant interleukin-1 beta (hrIL-1 beta) and the prostaglandin inhibitor indomethacin on the course of Cryptosporidium baileyi infection in chickens were studied. Daily oocyst shedding was monitored by a quantitative method throughout the experiment. Humoral immune response to C. baileyi was assessed by ELISA at 3 weeks of age while the level of cellular immune response to phytohaemagglutinin-P (PHA-P) by a skin test at 23 days of age. Parenteral application of hrIL-1 beta decreased oocyst shedding to 62%, but the infection ran a similar course in treated and control birds. The PHA-P skin test demonstrated increased cellular immune reaction in chickens receiving IL-1 beta, but there was no significant difference in the humoral responses of the two groups as detected by ELISA. On the other hand, indomethacin mixed to the feed lessened oocyst shedding to 13.7% and also shortened its duration. Immunological parameters as reflected by PHA-P skin test and ELISA results indicated enhanced cellular but unaltered humoral immune response. These data suggest that the systemic application of interleukin-1 can induce partial protection against C. baileyi in chickens and that prolonged, abundant oocyst shedding is due to an indomethacin-sensitive immunodepression via the prostaglandin pathway.
研究了人重组白细胞介素 -1β（hrIL-1β）和前列腺素抑制剂吲哚美辛对鸡贝氏隐孢子虫感染过程的影响。在整个实验过程中，通过定量方法监测每日卵囊排出情况。在3周龄时通过ELISA评估对贝氏隐孢子虫的体液免疫反应，而在23日龄时通过皮肤试验评估对植物血凝素 -P（PHA-P）的细胞免疫反应水平。皮下注射hrIL-1β可使卵囊排出减少至62%，但治疗组和对照组鸡的感染过程相似。PHA-P皮肤试验表明，接受IL-1β的鸡的细胞免疫反应增强，但ELISA检测显示两组的体液反应无显著差异。另一方面，添加到饲料中的吲哚美辛可使卵囊排出减少至13.7%，并缩短其持续时间。PHA-P皮肤试验和ELISA结果所反映的免疫参数表明细胞免疫增强，但体液免疫未改变。这些数据表明，全身性应用白细胞介素 -1可诱导鸡对贝氏隐孢子虫产生部分保护作用，且长时间大量排出卵囊是由于通过前列腺素途径的吲哚美辛敏感型免疫抑制所致。
