Ruelas Villavicencio A L, Aponte Paredes P S, Ortiz Hidalgo C
Departamento de Biología Celular y Tisular, Escuela de Medicina Universidad Panamericana.
Rev Invest Clin. 1999 Jan-Feb;51(1):11-6.
To explore the immunohistochemistry of elastofibromas and to evaluate four elastic strain methods for the histological diagnosis of elastofibroma.
Four elastofibromas were obtained from the surgical pathology files of the ABC Medical Center. All patients were women whose lesions were present in the subescapular region. Immunohistochemistry was performed for CD34, actin, desmin, vimentin, S-100 protein and bcl-2 and four elastic stain methods were evaluated (Verhoff, Gallego's, Reyes-Mota and Russel-Movat).
In the four cases there were numerous miofibroblasts (vimentin/actin/desmin positive). An unpreviously reported positive CD34 dendritic cells, were present diffusely in all four cases. Verhoff and Reyes-Mota stains remains the most useful methods to reveal the elastic fibers. Russel-Movat method may be use to contrast different connective tissue components.
The cell of elastofibromas were originally considered fibroblasts. The presence of actin/desmin/vimentin suggests that they are miofibroblasts. We describe for the first time, variable numbers of CD34(QBend/10) positive spindle shaped and dendritic cells in all elastofibromas. These cells may be a reactive population of cells of the "dendritic cell system". There was no immunoreactivity for bcl-2 in these cells.
探讨弹力纤维瘤的免疫组织化学特征，并评估四种弹性染色方法在弹力纤维瘤组织学诊断中的应用。
从ABC医疗中心的手术病理档案中获取4例弹力纤维瘤。所有患者均为女性，病变位于肩胛下区域。对CD34、肌动蛋白、结蛋白、波形蛋白、S-100蛋白和bcl-2进行免疫组织化学检测，并评估四种弹性染色方法（Verhoff法、加列戈法、雷耶斯-莫塔法和拉塞尔-莫瓦特法）。
4例病例中均有大量肌成纤维细胞（波形蛋白/肌动蛋白/结蛋白阳性）。在所有4例病例中均弥漫性存在一种此前未报道过的CD34阳性树突状细胞。Verhoff法和雷耶斯-莫塔法仍是显示弹性纤维最有用的方法。拉塞尔-莫瓦特法可用于对比不同的结缔组织成分。
弹力纤维瘤的细胞最初被认为是成纤维细胞。肌动蛋白/结蛋白/波形蛋白的存在表明它们是肌成纤维细胞。我们首次描述了在所有弹力纤维瘤中均存在数量不等的CD34（QBend/10）阳性梭形和树突状细胞。这些细胞可能是“树突状细胞系统”的反应性细胞群体。这些细胞中bcl-2无免疫反应性。
