Shaw A C, Christiansen G, Birkelund S
Department of Medical Microbiology and Immunology, University of Aarhus, Denmark.
Electrophoresis. 1999 Apr-May;20(4-5):775-80. doi: 10.1002/(SICI)1522-2683(19990101)20:4/5<775::AID-ELPS775>3.0.CO;2-Z.
Chlamydia trachomatis is an obligate intracellular bacterium causing human ocular and genital disease. The lymphokine interferon gamma (IFN-gamma) is an important immune effector exerting antimicrobial effects towards several intracellular parasites, the chlamydia included. IFN-gamma has been reported to inhibit the chlamydial replication in vitro in part by depleting intracellular levels of tryptophan in a dose-dependent manner. In addition, down-regulation of important immunogens has been described. These findings are extended in this paper, in which we are combining pulse labeling with [35S]methionine and two-dimensional gel electrophoresis with immobilized pH gradients in order to investigate changes in the protein expression of C. trachomatis serovar A and L2 caused by treatment with IFN-gamma. In contrast to what was observed in C. trachomatis L2, our results showed that, in C. trachomatis A, down-regulations of the chlamydia major outer membrane protein and of several other proteins were detectable upon IFN-gamma treatment. In addition, we report the up-regulations of C. trachomatis A and L2 proteins with molecular masses of approximately 30 kDa and 40 kDa which may be part of an, as yet, uncharacterized chlamydial response to IFN-gamma treatment.
沙眼衣原体是一种专性细胞内细菌，可引起人类眼部和生殖器疾病。淋巴因子干扰素γ（IFN-γ）是一种重要的免疫效应因子，对包括衣原体在内的几种细胞内寄生虫具有抗菌作用。据报道，IFN-γ在体外可通过以剂量依赖的方式消耗细胞内色氨酸水平来抑制衣原体复制。此外，还描述了重要免疫原的下调情况。本文扩展了这些发现，我们将[35S]甲硫氨酸脉冲标记与固定化pH梯度二维凝胶电泳相结合，以研究IFN-γ处理对沙眼衣原体血清型A和L2蛋白表达的影响。与沙眼衣原体L2中观察到的情况相反，我们的结果表明，在沙眼衣原体A中，IFN-γ处理后可检测到衣原体主要外膜蛋白和其他几种蛋白的下调。此外，我们报告了沙眼衣原体A和L2中分子量约为30 kDa和40 kDa的蛋白上调，这可能是衣原体对IFN-γ处理尚未明确的反应的一部分。
