Alaiya A A, Franzén B, Moberger B, Silfverswärd C, Linder S, Auer G
Department of Oncology-Pathology, Cancer Center Karolinska, Karolinska Institute and Hospital, Stockholm, Sweden.
Electrophoresis. 1999 Apr-May;20(4-5):1039-46. doi: 10.1002/(SICI)1522-2683(19990101)20:4/5<1039::AID-ELPS1039>3.0.CO;2-4.
The process of tumor progression leads to the emergence of multiple clones, and to the development of tumor heterogeneity. One approach to the study of the extent of such heterogeneity is to examine the expression of marker proteins in different tumor areas. Two-dimensional gel electrophoresis (2-DE) is a powerful tool for such studies, since the expression of a large number of polypeptide markers can be evaluated. In the present study, tumor cells were prepared from human ovarian tumors and analyzed by 2-DE and PDQUEST. As judged from the analysis of two different areas in each of nine ovarian tumors, the intratumoral variation in protein expression was low. In contrast, large differences were observed when the protein profiles of different tumors were compared. The differences in gene expression between pairs of malignant carcinomas were slightly larger than the differences observed between pairs of benign tumors. We conclude that 2-DE analysis of intratumoral heterogeneity in ovarian cancer tissue indicates a low degree of heterogeneity.
肿瘤进展过程会导致多个克隆的出现以及肿瘤异质性的发展。研究这种异质性程度的一种方法是检测不同肿瘤区域中标记蛋白的表达。二维凝胶电泳（2-DE）是进行此类研究的有力工具，因为可以评估大量多肽标记物的表达。在本研究中，从人卵巢肿瘤中制备肿瘤细胞，并通过2-DE和PDQUEST进行分析。根据对九个卵巢肿瘤中每个肿瘤的两个不同区域的分析判断，肿瘤内蛋白质表达的差异较小。相比之下，当比较不同肿瘤的蛋白质谱时，观察到了很大差异。恶性癌对之间的基因表达差异略大于良性肿瘤对之间观察到的差异。我们得出结论，对卵巢癌组织中肿瘤内异质性的2-DE分析表明异质性程度较低。
