Mitsuda H, Nakajima K
J Nutr Sci Vitaminol (Tokyo). 1976;22(4):307-12. doi: 10.3177/jnsv.22.307.
The addition of glyoxal, a trapping agent, caused simultaneously the accumulation of a green fluorescent compound and the inhibition of riboflavin formation in non-growing cells of Eremothecium ashbyii. The fluorescent compound purified was identified as 8-ribityllumazine from the results of spectrophotometric and fluorometric analyses. Accordingly, the fragment, except for the glyoxal portion on the 8-ribityllumazine molecule, 4-ribitylamino-5-amino-2,6-dihydroxypyrimidine, is believed to be an intermediate in the riboflavin biosynthetic pathway.
诱捕剂乙二醛的添加，同时导致了绿色荧光化合物的积累以及阿舒假囊酵母非生长细胞中核黄素形成的抑制。通过分光光度法和荧光分析法的结果，纯化得到的荧光化合物被鉴定为8-核醇基- lumazine。因此，除了8-核醇基- lumazine分子上的乙二醛部分外，片段4-核醇基氨基-5-氨基-2,6-二羟基嘧啶被认为是核黄素生物合成途径中的一个中间体。
