Volz J, Köster S, Spacek Z, Paweletz N
Department of Gynecology and Obstetrics, Universitäts-Klinikum Mannheim, D-68135 Mannheim, Germany.
Surg Endosc. 1999 Jun;13(6):611-4. doi: 10.1007/s004649901052.
Any route of entry into the abdomen contributes to alterations of the intraperitoneal organs with different clinical consequences. Characteristic alterations of the peritoneum after CO2 pneumoperitoneum used in laparoscopic surgery is examined.
A CO2 pneumoperitoneum with an intraperitoneal pressure of 6 mmHg was applied for 30 min in 32 nude mice. In the course of 4 days, the animals were killed and the peritoneal surface of the abdominal wall was studied by means of scanning electron microscopy.
Already 2 h after release of the pneumoperitoneum, mesothelial cells were bulging up. The intercellular clefts thereby increased in size, and the underlying basal lamina became visible. This reaction peaked after 12 h. Subsequently, peritoneal macrophages and lymphocytes filled all gaps, thereby recovering the basal lamina.
The morphologic integrity of the peritoneum is temporarily disturbed by a CO2 pneumoperitoneum.
进入腹部的任何途径都会导致腹腔内器官发生改变，并产生不同的临床后果。本研究对腹腔镜手术中使用的二氧化碳气腹后腹膜的特征性改变进行了检查。
对32只裸鼠施加腹腔内压力为6 mmHg的二氧化碳气腹30分钟。在4天的过程中，处死动物，并通过扫描电子显微镜研究腹壁的腹膜表面。
气腹解除后2小时，间皮细胞就开始隆起。细胞间缝隙因此增大，其下方的基膜变得可见。这种反应在12小时后达到峰值。随后，腹膜巨噬细胞和淋巴细胞填充了所有间隙，从而使基膜恢复。
二氧化碳气腹会暂时破坏腹膜的形态完整性。
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