Rudaz S, Veuthey J L
Chirality. 1999;11(4):319-25. doi: 10.1002/(SICI)1520-636X(1999)11:4<319::AID-CHIR10>3.0.CO;2-Y.
An extensive study of the behavior of three chiral stationary phases (CSP) is presented for the stereoselective determination of methadone. The following chromatographic columns were selected: a cellulose, Chiralcel OJ; a modified cyclodextrin. Cyclobond I 2000 RSP, and a protein, Chiral-AGP. Retention factors, enantioselectivity, efficiency, and resolution were tested by modifying the composition of the mobile phase as well as the temperature. The mechanism for the chiral recognition of methadone on each support was discussed. Optimal chromatographic parameters were obtained for the three supports tested, and methadone enantiomers were separated in less than 20 minutes. The cellulose-based column gave the best resolution, but this CSP was not adapted to clinical analyses of methadone. Under optimized conditions, the cyclodextrin- and protein-based columns allowed an excellent separation of methadone enantiomers, but no interference with the primary metabolite was found only with Chiral-AGP.
本文对三种手性固定相（CSP）用于美沙酮立体选择性测定的行为进行了广泛研究。选用了以下色谱柱：一种纤维素类的Chiralcel OJ；一种改性环糊精类的Cyclobond I 2000 RSP；以及一种蛋白质类的Chiral-AGP。通过改变流动相组成和温度来测试保留因子、对映体选择性、柱效和分离度。讨论了美沙酮在每种载体上的手性识别机制。对于所测试的三种载体都获得了最佳色谱参数，美沙酮对映体在不到20分钟内得以分离。纤维素基柱给出了最佳分离度，但这种CSP不适用于美沙酮的临床分析。在优化条件下，环糊精基柱和蛋白质基柱能实现美沙酮对映体的出色分离，但仅Chiral-AGP未发现对主要代谢物有干扰。
