集胞藻6803中锰ABC转运蛋白系统的跨膜蛋白组分MntB的膜拓扑结构。
Membrane topology of MntB, the transmembrane protein component of an ABC transporter system for manganese in the cyanobacterium Synechocystis sp. strain PCC 6803.
作者信息
Bartsevich V V, Pakrasi H B
机构信息
Department of Biology, Washington University, St. Louis, Missouri 63130, USA.
出版信息
J Bacteriol. 1999 Jun;181(11):3591-3. doi: 10.1128/JB.181.11.3591-3593.1999.
Abstract
The structure of the membrane protein MntB, a component of a manganese transporter system in Synechocystis sp. strain PCC 6803, was examined with a series of fusions to the reporter proteins alkaline phosphatase and beta-galactosidase. The results support a topological model for MntB consisting of nine transmembrane segments, with the amino terminus of the protein being in the periplasm and the carboxyl terminus being in the cytoplasm.
摘要
集胞藻PCC 6803锰转运系统的组成成分膜蛋白MntB的结构，通过与报告蛋白碱性磷酸酶和β-半乳糖苷酶的一系列融合进行了检测。结果支持了MntB的拓扑模型，该模型由九个跨膜区段组成，蛋白质的氨基末端位于周质中，羧基末端位于细胞质中。
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