Yu W, Shimoyama A, Uneda T, Obika S, Miyashita K, Doi T, Imanishi T
Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, 565-0871, Japan.
J Biochem. 1999 Jun;125(6):1034-8. doi: 10.1093/oxfordjournals.jbchem.a022383.
A monocationic lipid, YKS-220, with a symmetrical and biodegradable structure can be used as an effective gene transfer vector in a cationic particle form (not a cationic liposome form), and is obtained by diluting an ethanol solution of YKS-220 and DOPE (1:5, molar ratio) with an aqueous medium. This preparation method is more convenient than that for cationic liposomes. YKS-220 cationic particles showed a heterogeneous large mean diameter of 4.4 microm. An obvious size change was not observed when plasmid DNA was added. The transfection activity of YKS-220 cationic particles was comparable to those of YKS-220 liposomes and DOSPA liposomes (LipofectAMINE), and even higher than that of DOGS (TRNSFECTAM). Interestingly, the YKS-220 cationic particle/DNA complexes were resistant to the neutralizing effect of serum. All of these findings indicate that YKS-220 cationic particles are a convenient and efficient gene delivery reagent.
一种具有对称且可生物降解结构的单阳离子脂质YKS - 220，能够以阳离子颗粒形式（而非阳离子脂质体形式）用作有效的基因传递载体，它是通过用水性介质稀释YKS - 220与DOPE的乙醇溶液（摩尔比1:5）获得的。这种制备方法比制备阳离子脂质体的方法更简便。YKS - 220阳离子颗粒呈现出4.4微米的不均一较大平均直径。添加质粒DNA时未观察到明显的尺寸变化。YKS - 220阳离子颗粒的转染活性与YKS - 220脂质体和DOSPA脂质体（LipofectAMINE）相当，甚至高于DOGS（TRNSFECTAM）。有趣的是，YKS - 220阳离子颗粒/DNA复合物对血清的中和作用具有抗性。所有这些发现表明YKS - 220阳离子颗粒是一种方便且高效的基因递送试剂。
