Ohmiya R, Kato C, Yamada H, Aiba H, Mizuno T
Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University, Chikusa-ku, Nagoya, 464-8601, Japan.
J Biochem. 1999 Jun;125(6):1061-6. doi: 10.1093/oxfordjournals.jbchem.a022387.
An inspection of the Schizosaccharomyces pombe genome database revealed that this eukaryotic microorganism possesses a gene that may encode a bacterial type of histidine-to-aspartate (His-Asp) phosphorelay component, namely, a response regulator. The predicted gene, named prr1(+) (S. pombe response regulator), encodes a protein that contains a typical phospho-accepting receiver domain, preceded by a mammalian heat shock factor (HSF)-like DNA-binding domain. Inactivation of this prr1(+) gene resulted in mutant cells defective in some aspects of stress responses, including sensitivity to oxidative stress, cold-temperature, and heavy metal toxicity. It was also demonstrated that Prr1 is required for the transcription of some genes (e.g., trr1(+), ctt1(+)), which are induced by oxidative stress. These results suggest that a His-Asp phosphorelay system may be involved in a stress-activated signaling pathway in S. pombe.
对粟酒裂殖酵母基因组数据库的检查发现，这种真核微生物拥有一个可能编码细菌型组氨酸-天冬氨酸（His-Asp）磷酸化信号转导组分（即应答调节蛋白）的基因。这个预测的基因名为prr1(+)（粟酒裂殖酵母应答调节蛋白），编码一种蛋白质，该蛋白质含有一个典型的磷酸化接受结构域，其前面还有一个类似哺乳动物热休克因子（HSF）的DNA结合结构域。该prr1(+)基因的失活导致突变细胞在应激反应的某些方面存在缺陷，包括对氧化应激、低温和重金属毒性的敏感性。还证明了Prr1是一些受氧化应激诱导的基因（如trr1(+)、ctt1(+)）转录所必需的。这些结果表明，His-Asp磷酸化信号转导系统可能参与了粟酒裂殖酵母中的应激激活信号通路。
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