Aoyama K, Aiba H, Mizuno T
Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University, Japan.
Biosci Biotechnol Biochem. 2001 Oct;65(10):2347-52. doi: 10.1271/bbb.65.2347.
Common histidine-to-aspartate (His-to-Asp) phosphorelay signaling systems involve three types of signaling components: a sensor His-kinase, a response regulator, and a histidine-containing phosphotransfer (HPt) protein. In the fission yeast Schizosaccharomyces pombe, two response regulators, Mcs4 and Prr1, have been identified, and it was shown that they are involved in signal transduction in stress responses. Furthermore, Mcs4 and Prr1 appear to be involved in mitotic cell-cycle control and meiosis, respectively. Recently we have identified Spy1 (also known as Mpr1), which encodes an HPt phosphotransmitter, and reported that Spy1, together with Mcs4, plays a role in cell cycle regulation. In this study, we identified and characterized three genes encoding histidine kinase, named Phk1, Phk2, and Phk3 (S. pombe histidine kinase) (also referred as Mak2, Mak3, and Mak1, respectively). Deletion of individual kinase genes has no apparent phenotypes but multiple deletion of these kinases showed the same phenotype of Spyl (Mpr1)-deficient cells, indicating precocious entry into M phase. These results indicated that three histidine kinases that work upstream of the HPt-transmitter, Spy1 (Mpr1), have a redundant function in cell cycle control.
常见的组氨酸到天冬氨酸（His-to-Asp）磷酸化信号转导系统涉及三种信号转导成分：一种传感组氨酸激酶、一种应答调节蛋白和一种含组氨酸的磷酸转移（HPt）蛋白。在裂殖酵母粟酒裂殖酵母中，已鉴定出两种应答调节蛋白Mcs4和Prr1，并且已表明它们参与应激反应中的信号转导。此外，Mcs4和Prr1似乎分别参与有丝分裂细胞周期控制和减数分裂。最近，我们鉴定出了编码HPt磷酸传递体的Spy1（也称为Mpr1），并报道Spy1与Mcs4一起在细胞周期调控中发挥作用。在本研究中，我们鉴定并表征了三个编码组氨酸激酶的基因，命名为Phk1、Phk2和Phk3（粟酒裂殖酵母组氨酸激酶）（也分别称为Mak2、Mak3和Mak1）。单个激酶基因的缺失没有明显的表型，但这些激酶的多重缺失表现出与Spy1（Mpr1）缺陷细胞相同的表型，表明过早进入M期。这些结果表明，在HPt传递体Spy1（Mpr1）上游起作用的三种组氨酸激酶在细胞周期控制中具有冗余功能。
