Pinto G A, Vassallo J, Andrade L A, Magna L A
Department of Anatomical Pathology, Universidade Estadual de Campinas, São Paulo, Brazil.
Sao Paulo Med J. 1998 Nov-Dec;116(6):1846-51. doi: 10.1590/s1516-31801998000600004.
The integrity of basement membrane (BM) is damaged during the evolution of a benign or potentially malignant lesion into a malignant one, in which it may undergo several degrees of discontinuity as a necessary condition for the invasive process. Immunostaining for collagen IV, which is exclusively found in BM, has been used to evaluate its formation in neoplastic and benign lesions of several organs.
To investigate BM continuity pattern in squamous carcinoma "in situ" (CIS), microinvasive (MIC) and invasive (IC) squamous cell carcinoma of the uterine cervix, and to find out if BM expression could be useful in the diagnosis of early stromal invasion (MIC).
Archival material between 1988 and 1993 was studied at the Pathological Anatomy Department-Unicamp.
The selected cases, previously formalin fixed and paraffin embedded, were reviewed retrospectively by submitting them to immunohistochemical study via the avidin-biotin-peroxidase method using a monoclonal antibody anticollagen IV.
In all, 17 cases of CIS, 16 of MIC and 21 of IC were evaluated. All IC cases showed evident BM discontinuity, either focal or diffuse. In the CIS group, a continuous BM pattern was predominant, being focally disrupted in only 2/17 cases (11.8%). The MIC group showed an intermediate pattern, but with a clear tendency to BM discontinuity in 10/16 cases (62.5%). Inflammatory infiltrate, a variable also studied, cannot be considered responsible for BM discontinuity, since there was no statistical correlation between them.
We conclude that immunostaining for collagen IV may contribute to the diagnosis of stromal invasion by BM discontinuity.
在良性或潜在恶性病变演变为恶性病变的过程中，基底膜（BM）的完整性会受到破坏，在此过程中，基底膜可能会出现不同程度的连续性中断，这是侵袭过程的必要条件。仅在基底膜中发现的IV型胶原免疫染色已被用于评估其在多个器官的肿瘤性和良性病变中的形成情况。
研究子宫颈原位鳞状细胞癌（CIS）、微浸润（MIC）和浸润性（IC）鳞状细胞癌的基底膜连续性模式，并确定基底膜表达是否有助于早期间质浸润（MIC）的诊断。
对1988年至1993年期间坎皮纳斯大学病理解剖科的存档材料进行研究。
所选病例先前已用福尔马林固定并石蜡包埋，通过抗IV型胶原单克隆抗体采用抗生物素蛋白-生物素-过氧化物酶法进行免疫组织化学研究，对其进行回顾性分析。
共评估了17例CIS、16例MIC和21例IC病例。所有IC病例均显示明显的基底膜连续性中断，可为局灶性或弥漫性。在CIS组中，连续的基底膜模式占主导，仅2/17例（11.8%）出现局灶性破坏。MIC组显示出中间模式，但10/16例（62.5%）有明显的基底膜连续性中断倾向。还研究了炎症浸润这一变量，由于两者之间无统计学相关性，因此不能认为炎症浸润是基底膜连续性中断的原因。
我们得出结论，IV型胶原免疫染色可能有助于通过基底膜连续性中断诊断间质浸润。
