Baginsky M L, Brown W V
Physiol Chem Phys. 1976;8(3):197-206.
The previously demonstrated inhibition of cow's milk lipoprotein lipase by apoLp-Ala and the deinhibition by monoglyceride have been studied in more detail. The apoLp-Ala inhibition is reversible by the addition of monoglyceride before or after enzyme additions. Quantities of monoglyceride accumulate during hydrolysis of triglyceride which are adequate to prevent inhibition by added apoLp-Ala. Accelerating reaction rates observed when the substrate contains the apoprotein at levels producing partial inhibition are also explained by monoglyceride production. These effects were observed with both crude preparations of skim milk and highly pruified lipase. It is suggested that the generation of monoglyceride may be important in facilitating hydrolysis of triglyceride in lipoproteins containing this inhibitory apolipoprotein.
此前已证明载脂蛋白 - Ala对牛乳脂蛋白脂肪酶有抑制作用，而甘油单酯可使其解除抑制，对此进行了更详细的研究。在添加酶之前或之后加入甘油单酯，载脂蛋白 - Ala的抑制作用是可逆的。在甘油三酯水解过程中会积累一定量的甘油单酯，足以防止添加的载脂蛋白 - Ala产生抑制作用。当底物中载脂蛋白含量达到产生部分抑制的水平时观察到的反应速率加快现象，也可由甘油单酯的产生来解释。在脱脂乳粗制品和高度纯化的脂肪酶中均观察到了这些效应。有人提出，甘油单酯的生成可能在促进含有这种抑制性载脂蛋白的脂蛋白中甘油三酯的水解方面具有重要作用。
