Sweerts B W, Jarrott B, Lawrence A J
Department of Pharmacology, Monash University, Wellington Road, Clayton, Victoria 3168, Australia.
Brain Res Mol Brain Res. 1999 May 21;69(1):113-23. doi: 10.1016/s0169-328x(99)00095-9.
Exposure to stress is known to induce widespread changes in the central nervous system (CNS) involving multiple neuropeptides. The neuropeptide galanin has been implicated in the central response to different stressors; however, the role of galanin in the response to restraint stress has not been reported. Therefore, this study utilised in situ hybridisation histochemistry to observe the effects of acute and chronic restraint stress on preprogalanin (preproGAL) mRNA expression in the CNS of normotensive (Wistar Kyoto; WKY) and Spontaneously Hypertensive (SHR) rats. Rats were exposed to 1 h of restraint for 0 (control), 1, 3, 5, or 10 consecutive days, and central preproGAL mRNA expression following these restraint periods was compared between strains. Significant differences in the basal expression of preproGAL mRNA were detected, with expression decreased by approximately 50% in the supraoptic nucleus (SON; P<0. 01) and increased by approximately 100% in the rostral ventrolateral medulla (RVLM; P<0.05) of SHR when compared to WKY. Following acute restraint (1 session), preproGAL mRNA expression was significantly increased by approximately 135% in the central nucleus of the amygdala (CeA; P<0.05) in WKY. In SHR, significant increases of up to 300% were observed in the CeA (P<0.01) and SON (P<0.05) following chronic restraint (up to 10 days). In addition, expression of preproGAL mRNA was significantly decreased in the locus coeruleus (LC) of SHR following acute restraint (1 session) (P<0.05). These results provide the first evidence that both acute (LC) and chronic (CeA, SON) restraint stress is associated with alterations in preproGAL mRNA expression. As such, the present study provides further evidence linking neurons containing galanin with the central response to restraint stress.
已知暴露于应激会在中枢神经系统（CNS）中引发广泛变化，涉及多种神经肽。神经肽甘丙肽与对不同应激源的中枢反应有关；然而，甘丙肽在对束缚应激的反应中的作用尚未见报道。因此，本研究利用原位杂交组织化学观察急性和慢性束缚应激对正常血压（Wistar Kyoto；WKY）大鼠和自发性高血压（SHR）大鼠中枢神经系统中甘丙肽原（preproGAL）mRNA表达的影响。大鼠连续0（对照）、1、3、5或10天暴露于1小时的束缚，比较这些束缚期后两品系大鼠中枢preproGAL mRNA的表达。检测到preproGAL mRNA基础表达存在显著差异，与WKY相比，SHR的视上核（SON；P<0.01）中表达降低约50%，而延髓头端腹外侧区（RVLM；P<0.05）中表达增加约100%。急性束缚（1次）后，WKY大鼠杏仁核中央核（CeA；P<0.05）中preproGAL mRNA表达显著增加约135%。在SHR中，慢性束缚（长达10天）后，CeA（P<0.01）和SON（P<0.05）中观察到高达300%的显著增加。此外，急性束缚（1次）后，SHR大鼠蓝斑（LC）中preproGAL mRNA表达显著降低（P<0.05）。这些结果首次证明急性（LC）和慢性（CeA、SON）束缚应激均与preproGAL mRNA表达改变有关。因此，本研究为将含甘丙肽的神经元与对束缚应激的中枢反应联系起来提供了进一步证据。
