人类拓扑异构酶I与一种新型环指/精氨酸-丝氨酸蛋白之间的相互作用。
Interaction between human topoisomerase I and a novel RING finger/arginine-serine protein.
作者信息
Haluska P, Saleem A, Rasheed Z, Ahmed F, Su E W, Liu L F, Rubin E H
机构信息
Departments of Pharmacology and Medicine, Robert Wood Johnson Medical School, The Cancer Institute of New Jersey, University of Medicine and Dentistry of New Jersey, New Brunswick, NJ 08901, USA.
出版信息
Nucleic Acids Res. 1999 Jun 15;27(12):2538-44. doi: 10.1093/nar/27.12.2538.
The N-terminus of human topoisomerase I participates in the binding of this enzyme to helicases and other proteins. Using the N-terminal 250 amino acids of human topoisomerase I and a yeast two-hybrid/ in vitro binding screen, a novel arginine-serine-rich peptide was identified as a human topoisomerase I-binding protein. The corresponding full-length protein, named topors, contains a consensus RING zinc finger domain and nuclear localization signals in addition to the arginine-serine-rich region. The RING finger domain of topors is homologous to a similar domain in a family of viral proteins that are involved in the regulation of viral transcription. When expressed in HeLa cells as a green fluorescent protein fusion, topors localizes in the nucleus in a punctate pattern and co-immunoprecipitates with topoisomerase I. These data suggest that topors is involved in trans-cription, possibly recruiting topoisomerase I to RNA polymerase II transcriptional complexes.
人拓扑异构酶I的N端参与该酶与解旋酶及其他蛋白质的结合。利用人拓扑异构酶I的N端250个氨基酸以及酵母双杂交/体外结合筛选，一种新型富含精氨酸-丝氨酸的肽被鉴定为与人拓扑异构酶I结合的蛋白。相应的全长蛋白命名为topors，除富含精氨酸-丝氨酸区域外，还包含一个共有RING锌指结构域和核定位信号。topors的RING指结构域与一类参与病毒转录调控的病毒蛋白中的类似结构域同源。当作为绿色荧光蛋白融合体在HeLa细胞中表达时，topors以点状模式定位于细胞核，并与拓扑异构酶I进行共免疫沉淀。这些数据表明topors参与转录过程，可能将拓扑异构酶I招募至RNA聚合酶II转录复合物中。
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