Aránega A E, Velez C, Prados J, Melguizo C, Marchal J A, Arena N, Alvarez L, Aránega A
Department of Experimental Biology, University of Jaén, Spain.
Cells Tissues Organs. 1999;164(2):82-9. doi: 10.1159/000016645.
Early heart development is known to be sensitive to retinoid concentrations. Although the influence of retinoids on cardiac morphogenesis has been described previously, the effect of retinoids on cardiomyocyte differentiation during development has not been characterized. We quantified the effects of the retinoic acids all-trans RA and 13-cis RA on alpha-actin and alpha-actinin at the subcellular level in cultures of chick embryo cardiomyocytes obtained from Hamburger and Hamilton's (HH) stage 22, 32 and 40 embryos. The retinoids increased the concentration of alpha-actin and alpha-actinin in the cytoplasmic and cytoskeletal fractions of cells at all three stages of development. The effect was greatest in cardiomyocytes treated for 24 h with all-trans RA and in cells from HH22 embryos. The greatest increases in alpha-actin concentration occurred in the cytoskeletal fraction of HH22 cells cultured for 24 h with all-trans or 13-cis RA, whereas the greatest increases in alpha-actinin were found in the cytoplasmic fraction of HH22 cells exposed to retinoids for 24 h. We conclude that retinoic acid plays a role in the reorganization of the pattern of sarcomeric protein expression during cardiomyocyte differentiation.
已知心脏早期发育对类视黄醇浓度敏感。尽管此前已描述类视黄醇对心脏形态发生的影响，但类视黄醇在发育过程中对心肌细胞分化的作用尚未明确。我们对全反式视黄酸（all-trans RA）和13-顺式视黄酸（13-cis RA）对从汉伯格和汉密尔顿（HH）22、32和40期胚胎获取的鸡胚心肌细胞培养物中亚细胞水平的α-肌动蛋白和α-辅肌动蛋白的影响进行了量化。在所有三个发育阶段，类视黄醇均增加了细胞胞质和细胞骨架组分中α-肌动蛋白和α-辅肌动蛋白的浓度。在用全反式视黄酸处理24小时的心肌细胞以及来自HH22期胚胎的细胞中，这种作用最为明显。在用全反式或13-顺式视黄酸培养24小时的HH22细胞的细胞骨架组分中，α-肌动蛋白浓度增加最为显著，而在暴露于类视黄醇24小时的HH22细胞的胞质组分中，α-辅肌动蛋白增加最为显著。我们得出结论，视黄酸在心肌细胞分化过程中肌节蛋白表达模式的重组中发挥作用。
