Gurdina V V, Mel'nikova V A, Zaĭtsev T A, Osipov V V, Romanenko E E, Adlova G P, Baturo A P
Mechnikov Research Institute for Vaccines and Sera, Moscow, Russia.
Zh Mikrobiol Epidemiol Immunobiol. 1999 Mar-Apr(2):8-10.
Mini-pigs of the Svetlogorsk breed were immunized with vaccine prepared from of M. morganii culture; the culture had been grown in a liquid nutrient medium, obtained from casein hydrolysate, in flasks placed in a thermostatically controlled shaker to the phases of exponential growth and cell growth deceleration. O-agglutinating sera thus obtained were specific. The highest antibody level was detected in the serum obtained as the result of the immunization of guinea pigs with the vaccine prepared from the culture on the phase of exponential growth. This serum retained its properties after storage for 9 months. Heterologous antibodies occurred in the sera under study not more frequently than in rabbit sera and practically in the same titers.
用摩根氏菌培养物制备的疫苗对斯韦特洛戈尔斯克品种的小型猪进行免疫；该培养物在由酪蛋白水解物制成的液体营养培养基中，于置于恒温摇床中的烧瓶中培养至指数生长阶段和细胞生长减速阶段。由此获得的O凝集血清具有特异性。在用指数生长阶段的培养物制备的疫苗免疫豚鼠后获得的血清中检测到最高抗体水平。该血清在储存9个月后仍保持其特性。在所研究的血清中，异种抗体出现的频率不高于兔血清，且效价实际上相同。
