鹰嘴豆根瘤菌nod基因的诱导物。

Inducers of nod genes of Rhizobium ciceri.

作者信息

Srivastava P, Sharma P K, Dogra R C

机构信息

Department of Microbiology, College of Basic Sciences and Humanities, CCS Haryana Agricultural University, Hisar, India.

出版信息

Microbiol Res. 1999 May;154(1):49-55. doi: 10.1016/S0944-5013(99)80034-2.

DOI:10.1016/S0944-5013(99)80034-2

PMID:10356796

Abstract

Induction of nodABC genes of R. ciceri was studied by constructing nodABC-lacZ fusion. The root exudates of the homologous hosts induced the expression of nodABC genes but those of heterologous hosts failed to do so. The HPLC analysis of the root exudates of C. arietinum showed the presence of 6-7 compounds with retention times matching to flavonoids like naringenin, hesperetin, daidzein, naringin, 7 OH coumarin and luteolin. Induction studies using the standard flavonoids showed naringenin, followed by daidzein, as most potent inducer of the nodABC genes of R. ciceri. Naringenin in combination with daidzein showed a synergistic effect on the expression of nodABC genes.

摘要

通过构建nodABC-lacZ融合体研究了鹰嘴豆根瘤菌nodABC基因的诱导情况。同源宿主的根分泌物可诱导nodABC基因的表达，而异源宿主的根分泌物则不能。对鹰嘴豆根分泌物的高效液相色谱分析表明，存在6 - 7种化合物，其保留时间与黄酮类化合物如柚皮素、橙皮素、大豆苷元、柚皮苷、7-羟基香豆素和木犀草素相匹配。使用标准黄酮类化合物的诱导研究表明，柚皮素是鹰嘴豆根瘤菌nodABC基因最有效的诱导剂，其次是大豆苷元。柚皮素与大豆苷元组合对nodABC基因的表达具有协同作用。