Andersen CY, Morineau G, Fukuda M, Westergaard LG, Ingerslev HJ, Fiet J, Byskov AG
Laboratory of Reproductive Biology Section 5712, Juliane Marie Center for Children, Women and Reproduction, University Hospital of Copenhagen, Blegdamsvej 9, DK-2100, Copenhagen, Denmark, Laboratoire de Biologie Hormonale, Hopital Saint-Louis, 1.
Hum Reprod. 1999 Jun;14(6):1563-8. doi: 10.1093/humrep/14.6.1563.
Cortisol and cortisone concentrations in serum and follicular fluid (FF) from women undergoing in-vitro fertilization (IVF) treatment were monitored. Four groups were included: group 1, women in their natural menstrual cycle having an endogenous mid-cycle surge of gonadotrophins; group 2, women in their natural menstrual cycle receiving human chorionic gonadotrophin (HCG) for ovulation induction; group 3, women receiving exogenous gonadotrophins for ovarian stimulation and HCG for ovulation induction; and group 4, women receiving exogenous gonadotrophins for ovarian stimulation, follicles being aspirated immediately before administration of HCG. In this study, 12 follicles contained oocytes which resulted in clinical pregnancy after IVF. Cortisone concentrations were significantly higher in FF compared with that of matched serum samples, while the opposite was observed for cortisol, resulting in cortisol:cortisone ratios being significantly lower in FF compared with serum. FF from group 4 showed significantly higher cortisone concentrations than FF from each of the other three groups. FF from group 1 showed significantly higher cortisone concentrations and significantly lower cortisol:cortisone ratios in comparison with groups 2 and 3. None of the observed parameters pinpointed any of the follicles containing oocytes which resulted in a clinical pregnancy. The intrafollicular concentrations of cortisol and cortisone suggest that pre-ovulatory follicles actively convert cortisol to cortisone. Neither FF concentrations of cortisol and cortisone nor the cortisol:cortisone ratio seem to reflect implantation potential of the derived pre-embryos.
对接受体外受精（IVF）治疗的女性血清和卵泡液（FF）中的皮质醇和可的松浓度进行了监测。研究包括四组：第1组，处于自然月经周期且促性腺激素有内源性中期高峰的女性；第2组，处于自然月经周期并接受人绒毛膜促性腺激素（HCG）诱导排卵的女性；第3组，接受外源性促性腺激素刺激卵巢并接受HCG诱导排卵的女性；第4组，接受外源性促性腺激素刺激卵巢且在注射HCG前立即抽吸卵泡的女性。在本研究中，12个卵泡中的卵母细胞在IVF后导致临床妊娠。与匹配的血清样本相比，FF中的可的松浓度显著更高，而皮质醇则相反，导致FF中的皮质醇：可的松比值显著低于血清。第4组的FF显示可的松浓度显著高于其他三组中的任何一组。与第2组和第3组相比，第1组的FF显示可的松浓度显著更高，皮质醇：可的松比值显著更低。所观察到的参数均未明确指出任何含有导致临床妊娠的卵母细胞的卵泡。卵泡内皮质醇和可的松的浓度表明排卵前卵泡会将皮质醇主动转化为可的松。FF中皮质醇和可的松的浓度以及皮质醇：可的松比值似乎均不能反映所衍生的胚胎前体的着床潜力。
