Hidiroglou M, Wauthy J M, Proulx J E
Ann Rech Vet. 1976;7(2):185-94.
Two experiments to study nutritional muscular dystrophy (N.M.D.) in calves were conducted in northern Ontario, where the disease is prevalent. In the first experiment, ninety Shorthorn cows were used. They were divided into three groups and fed the following forages during the winter of 1972-1973. Group I - Silage. Group II. - Heated-air-dried hay. Group III. - Field-dried hay. Chemical analysis of the forage during storage showed that the silage had a higher tocopherol content than the other two forages. This higher content had a direct effect on plasma tocopherol concentrations in the cows, since tocopherol levels were found to be higher in the group fed silage than in the other two groups. The highest mortality rate-eight dead calves-was in the group fed heated-air-dried hay; one calf died in each of the other two groups. Hence, it is evident that the severity of N.M.D. symptoms in calves is directly linked with the quantity of selenium or vitamin E ingested. The substances act synergistically to protect against the disease. In a second experiment, a herd of forty-seven Shorthorn cows, some of which had calves with N.N.D. and some of which had healthy offspring, were studied to measure the activity of serum creatine phosphokinase. The dams were found to have the same C.P.K., whether or not their calves suffered from N.M.D.
在安大略省北部进行了两项研究犊牛营养性肌营养不良症（N.M.D.）的实验，该疾病在当地很普遍。在第一个实验中，使用了90头短角牛。它们被分成三组，并在1972 - 1973年冬季喂食以下草料。第一组 - 青贮饲料。第二组 - 热风干燥干草。第三组 - 田间干燥干草。储存期间对草料的化学分析表明，青贮饲料的生育酚含量高于其他两种草料。这种较高的含量对奶牛血浆生育酚浓度有直接影响，因为发现喂食青贮饲料的组中生育酚水平高于其他两组。死亡率最高的是喂食热风干燥干草的组，有8头犊牛死亡；其他两组各有1头犊牛死亡。因此，很明显犊牛中N.M.D.症状的严重程度与摄入的硒或维生素E的量直接相关。这些物质协同作用以预防该疾病。在第二个实验中，研究了一群47头短角牛，其中一些母牛的犊牛患有N.N.D.，一些母牛的后代健康，以测量血清肌酸磷酸激酶的活性。结果发现，无论母牛的犊牛是否患有N.M.D.，母牛的肌酸磷酸激酶水平都相同。
