Zaitseva G N, Mett I L, Kolesnikov A A
Biokhimiia. 1976 Aug;41(8):1406-11.
DNA-dependent RNA-polymerase activity was found in the kinetoplasts of Crithidia oncopelti. Kinetic patterns of 14C-UTP incorporation into the acid-insoluble fraction of isolated kinetoplasts at 25 degrees, 30 degrees and 35 degrees C were estimated. The effects of different antibiotics and intercalating agents on RNA synthesis in kinetoplasts were studied. alpha-amanitin, a specific inhibitor of the nuclear enzyme, does not affect the RNA-polymerase activity of the kinetoplasts. Streptolidigin and rifampicin, inhibitors of bacterial RNA-polymerase, have little effect on RNA synthesis in the kinetoplasts even at high concentrations. Kinetoplasts preincubation in the phosphate buffer increases the permeability of their membranes for rifampicin. Intercalating agents, acriflavin and ethidium bromide, strongly inhibit the kinetoplast RNA synthesis. Similar specific effects of some antibiotics and intercalating agents on RNA synthesis in kinetoplasts and typical mitochondria may be indicative of similarity of functional properties of RNA-polymerases in those organelles.
在罗得西亚锥虫的动基体中发现了依赖DNA的RNA聚合酶活性。估计了在25℃、30℃和35℃下，14C-UTP掺入分离的动基体酸不溶性部分的动力学模式。研究了不同抗生素和嵌入剂对动基体RNA合成的影响。α-鹅膏蕈碱是核酶的特异性抑制剂，不影响动基体的RNA聚合酶活性。细菌RNA聚合酶抑制剂链霉溶菌素和利福平即使在高浓度下对动基体中的RNA合成也几乎没有影响。在磷酸盐缓冲液中预孵育动基体可增加其膜对利福平的通透性。嵌入剂吖啶黄素和溴化乙锭强烈抑制动基体RNA合成。某些抗生素和嵌入剂对动基体和典型线粒体RNA合成的类似特异性作用可能表明这些细胞器中RNA聚合酶功能特性的相似性。
