Zaĭtseva G N, Mett I L, Maslov D A, Lunina L D, Koleshnkov A A
Biokhimiia. 1979 Nov;44(11):2073-82.
The coding properties of kinetoplast DNA from two respresentatives of the order Kinetoplastidae--Crithidia oncopelti and C. fasciculata--were studied. Experiments on hybridization of the whole network and fraction of minicircles with labelled 23S and 16S rRNA and with tRNA isolated from kinetoplasts of C. oncopelti clearly demonstrated the presence of the genes for these RNAs in the whole network and their absence in the minicircles. It may be thus concluded that the genes of ribosomal and transfer RNAs are localized in the maxicircular molecules. Similar efficiency of hybridization of rRNAs from C. oncopelti with kDNA from C. fasciculata and C. oncopelti revealed significant conservativity of ribosomal genes in the protozoa under study.
对动质体目两个代表物种——热带利什曼原虫和细滴虫——的动质体DNA的编码特性进行了研究。用标记的23S和16S rRNA以及从热带利什曼原虫动质体中分离的tRNA对整个网络和小环片段进行杂交实验，清楚地表明这些RNA的基因存在于整个网络中，而在小环中不存在。因此可以得出结论，核糖体RNA和转运RNA的基因定位于大环分子中。热带利什曼原虫的rRNA与细滴虫和热带利什曼原虫的kDNA杂交效率相似，表明在所研究的原生动物中核糖体基因具有显著的保守性。
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