Dovrat A, Weinreb O
B. Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa.
Invest Ophthalmol Vis Sci. 1999 Jun;40(7):1616-20.
To investigate the mechanisms involved in the damage caused by UV-A irradiation at 365 nm on the eye lens.
Bovine lenses obtained from animals 1 to 5 years of age were placed in specially designed organ culture chambers for preincubation. Twenty-four hours later, the lenses were irradiated by 33 J/cm2 UV-A at 365 nm. During irradiation, the lenses were oriented in the culture so that the anterior surface faced the incident UV-A radiation source. After irradiation, lens optical quality was monitored throughout the 8 days of the culture period, and lens samples were taken for analysis of NaK-ATPase activity.
Lens optics and NaK-ATPase activity were affected by irradiation of 33 J/cm2. The effects on lens epithelial NaK-ATPase activity were stronger at the equators than at the center. The damage to the activity at the center was reversible, as the lens optically recovered from the LW-A damage.
Lens NaK-ATPase activity can recover from damage caused by UV-A at 365 nm. When the lenses received irradiation of 33 J/cm2, NaK-ATPase activity recovered from the damage during the culture period only at the center and not at the equators of the epithelium.
研究365nm的UV-A辐射对眼晶状体造成损伤的相关机制。
将取自1至5岁动物的牛晶状体置于专门设计的器官培养室中进行预孵育。24小时后，用33J/cm²的365nm UV-A对晶状体进行照射。照射期间，将晶状体在培养中定向，使前表面面对入射的UV-A辐射源。照射后，在整个8天的培养期内监测晶状体的光学质量，并采集晶状体样本分析NaK-ATP酶活性。
33J/cm²的照射影响了晶状体光学和NaK-ATP酶活性。对晶状体上皮NaK-ATP酶活性的影响在赤道处比在中心更强。中心处活性的损伤是可逆的，因为晶状体从LW-A损伤中光学恢复。
晶状体NaK-ATP酶活性可从365nm的UV-A造成的损伤中恢复。当晶状体接受33J/cm²的照射时，NaK-ATP酶活性仅在培养期内在上皮中心而非赤道处从损伤中恢复。
