[肝细胞癌组织中谷胱甘肽S-转移酶M1基因的缺失突变]
[The mutation of deletion for glutathione S-transferase M1 gene in the tissue of hepatocellular carcinoma].
作者信息
Bian J, Shen F, Wang J, Chen G, Zhang B, Wu Y
机构信息
Department of Epidemiology, Shanghai Medical University, Shanghai, 200032 P.R.China.
出版信息
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 1999 Jun;16(3):171-3.
OBJECTIVE
To Study whether the mutation of deletion for glutathione S-transferase M1 (GSTM1) gene occurred during the development of hepatocellular carcinoma (HCC).
METHODS
The genotypes of GSTM1 of 46 pairs of HCC tissue and the noncancerous liver tissue were detected by polymerase chain reaction (PCR).
RESULTS
The frequency of GSTM1 null genotype for HCC tissue was 78.26%, but 65.22% for the noncancerous liver tissue (P<0.05). The GSTM1 null genotypes of 6 HCC tissue were transformed from the non- null genotypes of the noncancerous liver tissue. According to Hardy-Weinberg law., the rate of deletion mutation for GSTM1 gene was inferred to be 38.89%.
CONCLUSION
The results suggested that the mutation of deletion for GSTM1 gene had occurred during the development of HCC.
目的
研究谷胱甘肽S转移酶M1（GSTM1）基因缺失突变是否发生在肝细胞癌（HCC）的发生发展过程中。
方法
采用聚合酶链反应（PCR）检测46对HCC组织及癌旁肝组织的GSTM1基因型。
结果
HCC组织中GSTM1基因缺失基因型频率为78.26%，癌旁肝组织为65.22%（P<0.05）。6例HCC组织的GSTM1基因缺失基因型由癌旁肝组织的非缺失基因型转变而来。根据哈迪-温伯格定律，推断GSTM1基因缺失突变率为38.89%。
结论
结果提示GSTM1基因缺失突变发生在HCC的发生发展过程中。
Zotero 插件