Kasubuchi Y
No To Shinkei. 1976 Oct;28(10):1101-14.
Pregnant mice were injected intraperitoneally on different gestational day with either one or three successive doses of 30 mg/kg of cytosine arabinoside (ara-C), which has been known to interfere with DNA synthesis. Some of them were injected intraperitoneally with tritiated thymidine (3H-TdR) consecutively. The fetuses and the youngs were sacrificed at various hours and days after treatment. Pyknotic nuclei or nuclear debris were observed at the matrix layer three hours after the injection of single dose of ara-C. Nuclear debris were increased as time elapse and they were most prominent 12 hours after treatment. However, 24 hours later, new matrix layer was regenerating. Twenty-four hours after treatment with two successive doses of ara-C, labeling index in the matrix layer was about one third of that of control. Cerebral hemispheres of the treated youngs were reduced in size. The youngs, treated with three successive doses of ara-C on day 13, 14 and 15 of gestation, showed most severe microcephalus. Cytoarchitecture in the cortices of these microcephalic mice was characterized by irregular arrangement of the pyramidal neurons and their dendritic branches. Autoradiographic study revealed that cortical neurons which were produced at the regenerated matrix layer after ara-C treatment migrated to the surface of the cortex.
在不同的孕期天数，给怀孕小鼠腹腔注射30毫克/千克的阿糖胞苷（ara-C），剂量为单次或连续三次，已知该药物会干扰DNA合成。部分小鼠连续腹腔注射氚标记胸腺嘧啶核苷（3H-TdR）。在治疗后的不同时间点和天数处死胎儿和幼崽。注射单次剂量的ara-C三小时后，在基质层观察到固缩核或核碎片。随着时间推移，核碎片增多，治疗后12小时最为明显。然而，24小时后，新的基质层开始再生。连续两次注射ara-C治疗24小时后，基质层的标记指数约为对照组的三分之一。接受治疗的幼崽的大脑半球体积减小。在妊娠第13、14和15天连续三次注射ara-C的幼崽表现出最严重的小头畸形。这些小头畸形小鼠皮质的细胞结构特征为锥体细胞及其树突分支排列不规则。放射自显影研究显示，ara-C治疗后在再生基质层产生的皮质神经元迁移至皮质表面。
