Neĭfakh A A
Ontogenez. 1976;7(6):630-3.
The early embryos L. stagnalis were placed in the actinomycin solution at the successive developmental stages. The permeability to actinomycin was previously increased by the pricking through egg capsules. The inactivation of nuclei by actinomycin up to the stage of 12 blastomeres resulted in the arrest of development at the 22 cell stage. The inactivation of nuclei at the subsequent development stages resulted in the developmental arrest at later stages. These data suggest that the embryonic development up to the 22 cell stage is provided by the nuclear function during oogenesis. The morphogenetic nuclear function of the embryo begins at the stage of 12 blastomeres and provides the embryonic development beyond the 22 cell stage.
将早期的椎实螺胚胎置于放线菌素溶液中,处于连续的发育阶段。此前通过刺破卵囊增加了对放线菌素的通透性。在12个卵裂球阶段之前,放线菌素使细胞核失活导致发育在22细胞阶段停止。在随后的发育阶段细胞核失活导致在更晚阶段发育停止。这些数据表明,直至22细胞阶段的胚胎发育是由卵子发生期间的核功能提供的。胚胎的形态发生核功能始于12个卵裂球阶段,并为22细胞阶段之后的胚胎发育提供支持。