Belecky-Adams T L, Scheurer D, Adler R
Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, 21287, USA.
Dev Biol. 1999 Jun 1;210(1):107-23. doi: 10.1006/dbio.1999.9268.
We have investigated whether the activin family of growth factors is involved in the regulation of retinal cell differentiation. Immunocytochemistry and in situ hybridization have shown that activin/inhibin subunits alpha, betaA, and betaB; receptors II and IIB; follistatin; and a follistatin-like gene are expressed in different regions of the chick embryo retina in developmentally regulated patterns. When tested in dissociated retinal cultures, activin did not appear to affect cell survival or proliferation, but it exerted marked inhibitory effects on the differentiation of photoreceptors, while stimulating the differentiation of nonphotoreceptor neurons; both effects were concentration-dependent and follistatin-sensitive. The results are consistent with the possibility that activin family members play significant roles in the regulation of retinal development.
我们研究了生长因子激活素家族是否参与视网膜细胞分化的调节。免疫细胞化学和原位杂交表明，激活素/抑制素亚基α、βA和βB；受体II和IIB；卵泡抑素；以及一个类卵泡抑素基因，以发育调控模式在鸡胚视网膜的不同区域表达。在分离的视网膜培养物中进行测试时，激活素似乎不影响细胞存活或增殖，但它对光感受器的分化产生显著抑制作用，同时刺激非光感受器神经元的分化；这两种作用均呈浓度依赖性且对卵泡抑素敏感。这些结果与激活素家族成员在视网膜发育调节中发挥重要作用的可能性相一致。
