Cook P R
Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK.
Science. 1999 Jun 11;284(5421):1790-5. doi: 10.1126/science.284.5421.1790.
Models for replication and transcription often display polymerases that track like locomotives along their DNA templates. However, recent evidence supports an alternative model in which DNA and RNA polymerases are immobilized by attachment to larger structures, where they reel in their templates and extrude newly made nucleic acids. These polymerases do not act independently; they are concentrated in discrete "factories," where they work together on many different templates. Evidence for models involving tracking and immobile polymerases is reviewed.
复制和转录模型通常显示聚合酶像机车沿着DNA模板移动。然而，最近的证据支持另一种模型，即DNA和RNA聚合酶通过附着在更大的结构上而固定下来，在这些结构中它们卷入模板并挤出新合成的核酸。这些聚合酶并非独立起作用；它们集中在离散的“工厂”中，在那里它们共同作用于许多不同的模板。本文综述了涉及移动和固定聚合酶模型的证据。
