环氧化酶-1和环氧化酶-2信使核糖核酸及蛋白质在人脑和外周器官中的分布。
Distribution of cyclooxygenase-1 and cyclooxygenase-2 mRNAs and proteins in human brain and peripheral organs.
作者信息
Yasojima K, Schwab C, McGeer E G, McGeer P L
机构信息
Kinsmen Laboratory of Neurological Research, Department of Psychiatry, University of British Columbia, 2255 Westbrook Mall, Vancouver, B.C., Canada.
出版信息
Brain Res. 1999 Jun 5;830(2):226-36. doi: 10.1016/s0006-8993(99)01389-x.
We used the techniques of reverse transcriptase-polymerase chain reaction, Western blotting and immunohistochemistry to evaluate the expression of cyclooxygenase (COX)-1 and -2 in brain and peripheral organs of Alzheimer disease (AD) and control cases. We found both COX-1 and COX-2 to be constitutively expressed in all organs tested, i.e., brain, heart, liver, kidney, spleen and intestine. COX-2 was substantially upregulated in affected areas of AD brain and in infarcted areas of human heart. COX-1 was only mildly upregulated in AD brain. Immunohistochemically, COX-2 was strongly expressed in the perinuclear, dendritic and axonal areas of pyramidal neurons, with enhanced staining in AD. These data suggest a special role for COX-2 in neuronal function.
我们运用逆转录聚合酶链反应、蛋白质免疫印迹法和免疫组织化学技术，评估了阿尔茨海默病（AD）患者及对照者脑和外周器官中环氧合酶（COX）-1和COX-2的表达情况。我们发现COX-1和COX-2在所有检测器官（即脑、心脏、肝脏、肾脏、脾脏和肠道）中均有组成性表达。COX-2在AD脑的病变区域和人类心脏的梗死区域显著上调。COX-1在AD脑中仅轻度上调。免疫组织化学显示，COX-2在锥体细胞的核周、树突和轴突区域强烈表达，在AD中染色增强。这些数据表明COX-2在神经元功能中具有特殊作用。
Zotero 插件