Chase G W, Eitenmiller R R, Long A R
U.S. Food and Drug Administration, Atlanta Center for Nutrient Analysis, GA 30309, USA.
J AOAC Int. 1999 May-Jun;82(3):663-5.
A liquid chromatographic method is described for analysis of beta-carotene in medical food. The nutrient is extracted from medical food without saponification by matrix solid-phase dispersion and quantitated by isocratic normal-phase chromatography with a Si 60 column and a mobile phase of hexane containing 0.125% (v/v) isopropyl alcohol. The limit of quantitation is 0.02 microgram/mL at 436 nm. Standard response was linear over the concentration range of 0.02-1.0 microgram/mL (r2 = 0.99998). Recoveries were determined on a zero control reference material containing added beta-carotene at various levels. Recoveries averaged 91.2% (n = 25) with coefficients of variation from 0.50 to 3.10%. The method provides a rapid, specific, sensitive, and easily controlled assay for analysis of beta-carotene in fortified medical food. In addition, retinyl palmitate can be assayed simultaneously with an in-line fluorescence detector.
描述了一种用于分析医用食品中β-胡萝卜素的液相色谱方法。该营养素通过基质固相分散法从医用食品中提取，无需皂化，并采用Si 60柱和含0.125%（v/v）异丙醇的己烷流动相通过等度正相色谱法定量。在436 nm处的定量限为0.02微克/毫升。标准响应在0.02 - 1.0微克/毫升的浓度范围内呈线性（r2 = 0.99998）。在添加了不同水平β-胡萝卜素的零对照参考物质上测定回收率。回收率平均为91.2%（n = 25），变异系数为0.50%至3.10%。该方法为强化医用食品中β-胡萝卜素的分析提供了一种快速、特异、灵敏且易于控制的测定方法。此外，棕榈酸视黄酯可以用在线荧光检测器同时进行测定。
