Matsuoka M, Ito M, Takahashi K, Sakuragawa N
Thromb Haemost. 1976 Nov 30;36(2):441-50.
This paper presents the results of an immunological study of hemophilia B and its carriers which used two kinds of antibodies, a heterologous antibody of high specificity and a homologous antibody developed in a patient with severe hemophilia B. 1. The factor IX related antigen for hemophilia B could be determined by a neutralization test and not by a precipitation reaction. 2. The activity of factor IX in a definite carrier of hemophilia B was significantly lower than that of factor VIII in a definite carrier of hemophilia A. 3. In hemophilia B, there were no differences between the factor IX antigen determined by either the heterologous rabbit antibodies or the homologous antibodies. And there was no discrepancy between the factor IX antigen and the factor IX activity. Therefore, we cannot use this discrepancy for the detection of a carrier of hemophilia B. 4. However, there was a discrepancy between the factor IX antigen determined by the neutralization test and the factor IX procoagulant activity in the patients of both hemophilia BM and hemophilia B+. The identical results were obtained in the cases of carriers of both hemophilia BM and hemophilia B+. These facts are very useful for the detection of carriers of hemophilia BM and hemophilia B+.
本文介绍了一项针对乙型血友病及其携带者的免疫学研究结果，该研究使用了两种抗体，一种是高特异性的异源抗体，另一种是在一名重型乙型血友病患者体内产生的同源抗体。1. 乙型血友病的因子IX相关抗原可通过中和试验而非沉淀反应来测定。2. 一名明确的乙型血友病携带者体内因子IX的活性显著低于一名明确的甲型血友病携带者体内因子VIII的活性。3. 在乙型血友病中，用异源兔抗体或同源抗体测定的因子IX抗原之间没有差异。并且因子IX抗原与因子IX活性之间没有偏差。因此，我们不能利用这种偏差来检测乙型血友病携带者。4. 然而，在BM型乙型血友病和B+型乙型血友病患者中，通过中和试验测定的因子IX抗原与因子IX促凝活性之间存在偏差。在BM型乙型血友病和B+型乙型血友病携带者的病例中也获得了相同的结果。这些事实对于检测BM型乙型血友病和B+型乙型血友病携带者非常有用。
