Jin C, Marsden I, Chen X, Liao X
Department or Biochemistry and Molecular Biology, College of Medicine, University of Illinois at Chicago, Chicago, IL, 60612, USA.
J Mol Biol. 1999 Jun 18;289(4):683-90. doi: 10.1006/jmbi.1999.2819.
Genesis is an HNF-3/fkh homologous protein. By using multi-dimensional NMR techniques, we have obtained the solution structure and backbone dynamics of Genesis complexed with a 17 base-pair DNA. Our results indicate that both the local folding and dynamic properties of Genesis are perturbed when it binds to the DNA site. Our data show that a conserved flexible amino acid sequence (wing 1) makes dynamic contacts to DNA in the complex and a short helix is induced by Genesis-DNA interactions. Our data indicate that, unlike the HNF-3gamma/DNA complex, a magnesium ion is not required in forming the stable Genesis-DNA complex.
Genesis是一种HNF-3/fkh同源蛋白。通过使用多维核磁共振技术，我们获得了与一段17个碱基对的DNA结合的Genesis的溶液结构和主链动力学。我们的结果表明，当Genesis与DNA位点结合时，其局部折叠和动力学特性均受到干扰。我们的数据显示，一个保守的柔性氨基酸序列（翼1）在复合物中与DNA进行动态接触，并且Genesis与DNA的相互作用诱导形成了一个短螺旋。我们的数据表明，与HNF-3γ/DNA复合物不同，形成稳定的Genesis-DNA复合物不需要镁离子。
