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光学图谱及其在大规模测序项目中的潜力。

Optical mapping and its potential for large-scale sequencing projects.

作者信息

Aston C, Mishra B, Schwartz D C

机构信息

Wyeth-Ayerst Research, CNS Disorders, Princeton, NJ 08543, USA.

出版信息

Trends Biotechnol. 1999 Jul;17(7):297-302. doi: 10.1016/s0167-7799(99)01326-8.

DOI:10.1016/s0167-7799(99)01326-8
PMID:10370237
Abstract

Physical mapping has been rediscovered as an important component of large-scale sequencing projects. Restriction maps provide landmark sequences at defined intervals, and high-resolution restriction maps can be assembled from ensembles of single molecules by optical means. Such optical maps can be constructed from both large-insert clones and genomic DNA, and are used as a scaffold for accurately aligning sequence contigs generated by shotgun sequencing.

摘要

物理图谱已被重新发现，成为大规模测序项目的一个重要组成部分。限制性图谱以规定的间隔提供标志性序列，高分辨率限制性图谱可通过光学手段从单分子集合中组装而成。这种光学图谱既可以从大插入片段克隆构建，也可以从基因组DNA构建，并用作支架，用于精确比对鸟枪法测序产生的序列重叠群。

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