RAG1和RAG2四聚体复合物在V(D)J重组的切割过程中具有活性。
A RAG1 and RAG2 tetramer complex is active in cleavage in V(D)J recombination.
作者信息
Bailin T, Mo X, Sadofsky M J
机构信息
Program of Gene Regulation, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, Georgia 30912-2650, USA.
出版信息
Mol Cell Biol. 1999 Jul;19(7):4664-71. doi: 10.1128/MCB.19.7.4664.
Abstract
During V(D)J recombination two proteins, RAG1 and RAG2, assemble as a protein-DNA complex with the appropriate DNA targets containing recombination signal sequences (RSSs). The properties of this complex require a fairly elaborate set of protein-protein and protein-DNA contacts. Here we show that a purified derivative of RAG1, without DNA, exists predominantly as a homodimer. A RAG2 derivative alone has monomer, dimer, and larger forms. The coexpressed RAG1 and RAG2 proteins form a mixed tetramer in solution which contains two molecules of each protein. The same tetramer of RAG1 and RAG2 plus one DNA molecule is the form active in cleavage. Additionally, we show that both DNA products following cleavage can still be held together in a stable protein-DNA complex.
摘要
在V(D)J重组过程中，两种蛋白质RAG1和RAG2与含有重组信号序列（RSSs）的合适DNA靶标组装成蛋白质-DNA复合物。该复合物的特性需要相当复杂的一组蛋白质-蛋白质和蛋白质-DNA相互作用。我们在此表明，一种不含DNA的RAG1纯化衍生物主要以同二聚体形式存在。单独的RAG2衍生物有单体、二聚体和更大的形式。共表达的RAG1和RAG2蛋白在溶液中形成混合四聚体，其中每种蛋白各有两个分子。RAG1和RAG2的相同四聚体加上一个DNA分子是具有切割活性的形式。此外，我们表明切割后的两种DNA产物仍可在稳定的蛋白质-DNA复合物中结合在一起。
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