Zhou Z, Liu W, Chen W
Department of Gastroenterology, Southwest Hospital, Third Military Medical College, Chongqing.
Zhonghua Nei Ke Za Zhi. 1997 Apr;36(4):225-7.
We evaluated the possibility of inducing a productive transfer of the IL-2 gene into human gastic cancer cells (GCC) and assessed the phenotypic and proliferative changes generated in the nude mice. The plasmid vector (BMGNeo-IL-2) carrying the human IL-2 gene was used to transduce the SGC-7901 GCC line by the lipofectin reagent. The IL-2 gene was analyzed by Southern blot and productive IL-2 release using IL-2-dependent CTLL. Cytotoxicity of LAK cells was tested by MTT colorimetry. The kinetics of in vitro growth and proliferation of parental and engineered cells were also measured. Parental and IL-2 gene transduced GCC were injected into nude mice. The tumorigenic potential of IL-2 gene-transfected GCC was evaluated by examining their in vivo growth in nude mice. The productive insertion of the IL-2 gene was achieved in SGC-7901. The amounts of IL-2 constitutively released by the engineered neoplastic cells ranged from 20 to 131 U/ml of IL-2 produced from 10(6) cells in 24 hours. Transduction of GCC with IL-2 gene did not modify the morphology and growth rate. IL-2 gene transfected cells demonstrated increased susceptibility to cell killing by LAK cells. IL-2 producing cells lost their tumorigenicity as evidenced by failure to grow in nude mice. The results demonstrate that IL-2 gene can be productively transduced into human gastric cancer cells without modifying their morphology and growth rate and this transduction leads to reduced or abrogated in vivo tumorigenic potential.
我们评估了将白细胞介素-2（IL-2）基因有效导入人胃癌细胞（GCC）的可能性，并评估了在裸鼠体内产生的表型和增殖变化。携带人IL-2基因的质粒载体（BMGNeo-IL-2）通过脂质体转染试剂转导SGC-7901 GCC细胞系。通过Southern印迹分析IL-2基因，并使用依赖IL-2的CTLL检测IL-2的有效释放。通过MTT比色法检测LAK细胞的细胞毒性。还测量了亲本细胞和工程细胞的体外生长和增殖动力学。将亲本GCC和转导了IL-2基因的GCC注射到裸鼠体内。通过检查它们在裸鼠体内的生长情况来评估转染了IL-2基因的GCC的致瘤潜力。在SGC-7901中实现了IL-2基因的有效插入。工程化肿瘤细胞组成性释放的IL-2量在每24小时从10⁶个细胞产生20至131 U/ml的IL-2范围内。用IL-2基因转导GCC并未改变其形态和生长速率。转染了IL-2基因的细胞对LAK细胞杀伤的敏感性增加。产生IL-2的细胞失去了致瘤性，这在裸鼠中未能生长得到证实。结果表明，IL-2基因可以有效转导到人胃癌细胞中，而不改变其形态和生长速率，并且这种转导导致体内致瘤潜力降低或消除。
