去甲二氢愈创木酸对SHG-44胶质瘤细胞系生长和分化的影响

[Effects of nordihydroguaiaretic acid on the growth and differentiation of SHG-44 glioma cell line].

作者信息

Bian X, Shi J, Xin R

机构信息

Department of Pathology, Third Military Medical University, Chongqing.

出版信息

Zhonghua Bing Li Xue Za Zhi. 1997 Oct;26(5):285-8.

PMID:10374337

Abstract

OBJECTIVE

To investigate the effects of nordihydroguaiaretic acid (NDGA) on the growth and differentiation of glioma cells and their mechanism.

METHODS

A human glioblastoma cell line (SHG-44) was treated with NDGA in the medium or intracytoplasmic microinjection of NDGA. Changes of the growth, morphology, cell cycle and immunohistochemical features of the cell were investigated.

RESULTS

It was found that the growth rates and proliferation activity were inhibited by 100 mumol/L NDGA in the medium, with cell cycles altered. NDGA could induce differentiation of these cells with the glial filaments increased, and the treated cells expressed more GFAP but less vimentin. The expression of p53 and bFGF were also decreased. Furthermore, the result of microinjection (1.5 x 10(-11) g/cell) showed similar but more rapid and permanent effects on the glioma cells.

CONCLUSION

NDGA has multiple effects of growth inhibition, differentiation therapy, as well as angiosuppression on human glioblastoma cells.

摘要

目的

探讨去甲二氢愈创木酸（NDGA）对胶质瘤细胞生长、分化的影响及其机制。

方法

采用培养基中加入NDGA或胞质内微量注射NDGA处理人胶质母细胞瘤细胞系（SHG-44），观察细胞生长、形态、细胞周期及免疫组化特征的变化。

结果

发现培养基中100μmol/L NDGA可抑制细胞生长速率和增殖活性，使细胞周期发生改变。NDGA可诱导这些细胞分化，胶质纤维增加，处理后的细胞表达更多的胶质纤维酸性蛋白（GFAP）而波形蛋白减少。p53和碱性成纤维细胞生长因子（bFGF）的表达也降低。此外，微量注射（1.5×10⁻¹¹ g/细胞）结果显示对胶质瘤细胞有相似但更快速和持久的作用。