Tayal A K, Das L, Kaur I
Department of Biosciences and Biotechnology, University of Roorkee, India.
Biomed Chromatogr. 1999 May;13(3):220-4. doi: 10.1002/(SICI)1099-0801(199905)13:3<220::AID-BMC823>3.0.CO;2-E.
White rot fungal strains screened from local sources (wood trunks and from effluents of pulp and paper industry) were tested for their ability to biodegrade polymeric compounds, viz. polymeric dyes (crystal violet and brilliant green) and chlorinated phenol (pentachlorophenol). Two of the most promising strains showing maximum degradation of polymeric dyes were selected to study the biodegradation potential and pattern of biodegradation of pentachlorophenol (PCP), a commonly used leather preservative and a potential carcinogen. PCP was quantitatively estimated and analysed by high-performance liquid chromatography (HPLC). Conditions were optimized for the measurement of PCP on HPLC, which were: mobile phase, 60% acetonitrile and 40% water; flow rate, 1 mL/ min; column, mu Bondapack C18 RP and UV detector at 238 nm. One of the white rot fungal strains isolated from wood trunk showed a maximum 68% biodegradation of PCP in liquid-buffered medium in 16 days. The biodegradation pattern of PCP followed a pseudo-first-order kinetics. Studies on enhancement of biodegradation of polymeric dyes and PCP showed that the kinetics of biodegradation is greatly improved by the presence of manganese ions, H2O2 and glucose in the medium. This strongly suggests the involvement of peroxidase enzyme machinery of white rot fungus in the biodegradation process of PCP.
对从当地来源（树干以及制浆造纸工业废水）筛选出的白腐真菌菌株进行了测试，以考察它们对聚合化合物的生物降解能力，即聚合染料（结晶紫和亮绿）和氯代酚（五氯苯酚）。选择了两种对聚合染料降解能力最强的最有前景的菌株，来研究五氯苯酚（PCP，一种常用的皮革防腐剂和潜在致癌物）的生物降解潜力及生物降解模式。通过高效液相色谱法（HPLC）对五氯苯酚进行定量估算和分析。对HPLC测定五氯苯酚的条件进行了优化，具体如下：流动相为60%乙腈和40%水；流速为1 mL/分钟；色谱柱为μ Bondapack C18 RP，紫外检测器波长为238 nm。从树干分离出的一种白腐真菌菌株在液体缓冲培养基中16天内对五氯苯酚的最大生物降解率为68%。五氯苯酚的生物降解模式符合准一级动力学。对聚合染料和五氯苯酚生物降解增强的研究表明，培养基中存在锰离子、过氧化氢和葡萄糖可大大改善生物降解动力学。这有力地表明白腐真菌的过氧化物酶机制参与了五氯苯酚的生物降解过程。
