Evertsson S, Bartik Z, Zhang H, Jansson A, Sun X F
Division of Oncology, Department of Biomedicine and Surgery, Linkoping University, S-581 85 Linkoping, Sweden.
Int J Oncol. 1999 Jul;15(1):53-8. doi: 10.3892/ijo.15.1.53.
Colorectal cancer is a disease that is associated with default in the balance of apoptotic regulation. In the present study apoptosis was examined in 158 colorectal adenocarcinomas using the terminal deoxynucleotidyl transferase mediated digoxigenin nick end labeling (TUNEL) method. The median apoptotic index (AI) was 0.95% (range 0-6. 68%). Eighty-two tumours exhibited AI </=0.95% and 76 tumours showed AI >0.95%. We revealed a positive correlation between apoptosis and proliferation determined as the expression of proliferating cell nuclear antigen (PCNA, p=0.002). The frequency of apoptosis increased from Dukes' stage A, B, C to D (p=0.01). No correlations were found between apoptosis and the patients' sex, age, tumour location, growth pattern, differentiation, prognosis, bcl-2, p53 or K-ras. Our findings suggest that we should further investigate the relationship between apoptosis and cellular proliferative activity in colorectal cancer to evaluate whether this might provide additional information in the selection of patients for effective adjuvant therapy.
结直肠癌是一种与凋亡调控平衡失调相关的疾病。在本研究中，采用末端脱氧核苷酸转移酶介导的地高辛配基缺口末端标记法（TUNEL法）对158例结直肠腺癌的凋亡情况进行了检测。凋亡指数（AI）中位数为0.95%（范围0 - 6.68%）。82例肿瘤的AI≤0.95%，76例肿瘤的AI>0.95%。我们发现凋亡与作为增殖细胞核抗原（PCNA）表达所确定的增殖之间呈正相关（p = 0.002）。凋亡频率从杜克分期A、B、C到D期逐渐增加（p = 0.01）。未发现凋亡与患者性别、年龄、肿瘤位置、生长方式、分化程度、预后、bcl - 2、p53或K - ras之间存在相关性。我们的研究结果表明，应进一步研究结直肠癌中凋亡与细胞增殖活性之间的关系，以评估这是否可为有效辅助治疗患者的选择提供额外信息。
