Dhillon H S, Prasad R M
Department of Surgery, University of Kentucky Chandler Medical Center, Lexington, KY 40536, USA.
Brain Res. 1999 Jun 19;832(1-2):7-12. doi: 10.1016/s0006-8993(99)01437-7.
This study examined the effects of the administration of kynurenate, a non-specific excitatory amino acid (EAA) receptor subtype antagonist, on the regional accumulation of diacylglycerol (DG) and free fatty acids (FFAs) after lateral fluid percussion (FP) brain injury in the rat. After brain injury of moderate severity (2.0 atm), rats were treated with either kynurenate (200 mg/kg, i.v.) or saline at 5 min after injury. In the saline-treated brain-injured rats, levels of all individual DG-fatty acids (palmitic, stearic, oleic and arachidonic acids) and total DG-fatty acids were increased in the ipsilateral left cortex and hippocampus at 30 min and 60 min after injury. Kynurenate administration attenuated increases of individual and total DG-fatty acids in the ipsilateral cortex at 30 and 60 min and in the ipsilateral hippocampus at 30 min after FP brain injury. At 30 and 60 min after FP brain injury, increases in the levels of individual FFAs (palmitic, stearic, oleic and arachidonic acids) and of total FFAs were also observed in the ipsilateral cortex and hippocampus of the saline-treated injured rats. Kynurenate administration attenuated increases of all individual and total FFAs in the ipsilateral cortex and hippocampus either at 30 min alone or at both 30 min and 60 min after FP brain injury. In the contralateral cortex, levels of both DG-fatty acids and FFAs were not increased in the saline-treated injured rats and were also not affected by the administration of kynurenate. These results support the role of EAA receptor subtypes in the phospholipases-catalyzed formation of DG and FFAs in the ipsilateral cortex and hippocampus after lateral FP brain injury.
本研究检测了非特异性兴奋性氨基酸（EAA）受体亚型拮抗剂犬尿酸盐对大鼠侧脑室液压冲击（FP）脑损伤后二酰基甘油（DG）和游离脂肪酸（FFA）区域蓄积的影响。在中度严重脑损伤（2.0个大气压）后，大鼠在损伤后5分钟接受犬尿酸盐（200mg/kg，静脉注射）或生理盐水治疗。在生理盐水治疗的脑损伤大鼠中，损伤后30分钟和60分钟时，同侧左皮质和海马中所有单个DG脂肪酸（棕榈酸、硬脂酸、油酸和花生四烯酸）及总DG脂肪酸水平均升高。给予犬尿酸盐可减轻FP脑损伤后30分钟和60分钟时同侧皮质以及30分钟时同侧海马中单个和总DG脂肪酸的升高。在FP脑损伤后30分钟和60分钟时，生理盐水治疗的损伤大鼠同侧皮质和海马中单个FFA（棕榈酸、硬脂酸、油酸和花生四烯酸）及总FFA水平也升高。给予犬尿酸盐可减轻FP脑损伤后仅30分钟时或30分钟和60分钟时同侧皮质和海马中所有单个和总FFA的升高。在对侧皮质中，生理盐水治疗的损伤大鼠中DG脂肪酸和FFA水平均未升高，且给予犬尿酸盐也未对其产生影响。这些结果支持了EAA受体亚型在侧脑室FP脑损伤后同侧皮质和海马中磷脂酶催化的DG和FFA形成中的作用。
