短暂性全脑缺血后大鼠海马肿瘤坏死因子-α免疫反应性细胞的超微结构定位

Ultrastructural localization of hippocampal TNF-alpha immunoreactive cells in rats following transient global ischemia.

作者信息

Tseng M T, Chang C C

机构信息

Department of Anatomical Sciences and Neurobiology, School of Medicine, University of Louisville, Louisville, KY 40292, USA.

出版信息

Brain Res. 1999 Jun 26;833(1):121-4. doi: 10.1016/s0006-8993(99)01490-0.

DOI:10.1016/s0006-8993(99)01490-0

PMID:10375686

Abstract

Using a polyclonal antibody against rat TNF-alpha, we have identified specific intracellular immunoreactive sites in hippocampal pyramidal cells, astroglia, and in microglia within 72 h after a period of ischemia. Electron opaque immunoreactive products in pyramidal cells were found mainly in somata and dendrites. Astrocytes and microglia were nearly devoid of such complexes. These findings demonstrate the presence of TNF-alpha in hippocampal neurons and its enhancement by ischemic stress.

摘要

利用抗大鼠肿瘤坏死因子-α（TNF-α）的多克隆抗体，我们在缺血一段时间后的72小时内，在海马锥体细胞、星形胶质细胞和小胶质细胞中鉴定出了特定的细胞内免疫反应位点。锥体细胞中的电子不透明免疫反应产物主要见于胞体和树突。星形胶质细胞和小胶质细胞几乎没有此类复合物。这些发现证明了海马神经元中存在TNF-α，并且缺血应激会使其增加。