ATP和S-140对樟脑毒素、蓖麻毒素及其他RNA N-糖苷酶导致核糖体失活的促进作用。
Promotion of ATP and S-140 to ribosome inactivation with camphorin, cinnamomin, and other RNA N-glycosidases.
作者信息
Ruan J P, Chen W F, Liu W Y
机构信息
Shanghai Institute of Biochemistry, Chinese Academy of Sciences, China.
出版信息
Zhongguo Yao Li Xue Bao. 1998 May;19(3):261-4.
AIM
To study the effect of ATP and extra-ribosomal factors (S-140) on type I and type II RNA N-glycosidases in inactivating ribosome.
METHODS
The activity of ATP and S-140 was determined by characterization of R-fragment in gel. An improved two-step method of cell-free protein synthesis system was used to quantitate the requirements of S-140 in ribosome inactivation.
RESULTS
IC50 ratios of camphorin, gamma-momorcharin, luffin S, luffin A, trichosanthin (type I); and ricin, ricin A-chain; cinnamonin, cinnamomin A-chain (type II) between the absence and presence of ATP and S-140 were 3108, 151, 51, 45, 15; and 47, 7, 26, 12, respectively.
CONCLUSION
The ribosome-inactivating activity of type II ribosome-inactivating proteins, including intact protein and its A-chain, was promoted by ATP and S-140. Camphorin showed a significant difference from cinnamomin in need of ATP and S-140 for such promoting.
目的
研究三磷酸腺苷（ATP）和核糖体外因子（S - 140）对Ⅰ型和Ⅱ型RNA N - 糖苷酶使核糖体失活的影响。
方法
通过凝胶中R片段的特性来测定ATP和S - 140的活性。采用改进的无细胞蛋白质合成系统两步法来定量S - 140在核糖体失活中的需求。
结果
在不存在和存在ATP及S - 140的情况下，Ⅰ型的樟芝毒素、γ - 苦瓜素、丝瓜毒素S、丝瓜毒素A、天花粉蛋白；以及Ⅱ型的蓖麻毒素、蓖麻毒素A链；肉桂毒素、肉桂毒素A链的半数抑制浓度（IC50）比值分别为3108、151、51、45、15；以及47、7、26、12。
结论
ATP和S - 140可促进包括完整蛋白及其A链在内的Ⅱ型核糖体失活蛋白的核糖体失活活性。樟芝毒素在需要ATP和S - 140来实现这种促进作用方面与肉桂毒素存在显著差异。
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