白细胞介素-1阻滞剂CK-119和CK-122对成纤维细胞样细胞增殖的抑制作用。

Inhibition of fibroblast-like cell proliferation by interleukin-1 blockers, CK-119 and CK-122.

作者信息

Bo X, Chiou G C

机构信息

Institute of Ocular Pharmacology, TX A&M University College of Medicine, College Station 77843-1114, USA.

出版信息

Zhongguo Yao Li Xue Bao. 1998 Jul;19(4):304-8.

PMID:10375772

Abstract

AIM

To study potent and nontoxic agents to inhibit fibroblast proliferation.

METHODS

Fibroblast-like corneal and conjunctival cells were cultured and inhibited by interleukin-1 (IL-1) blockers, dihydropyridazino-pyridazines CK-119 and CK-122. The cell growth and syntheses of DNA, RNA, and protein after IL-1 blocker incubation were determined.

RESULTS

CK-119 and CK-122 inhibited cell growth of corneal fibroblast at 30 mg.L-1. DNA and RNA syntheses in corneal fibroblasts were markedly inhibited by CK-119 and CK-122 whereas protein synthesis was either unaffected or mostly enhanced at 30-100 mg.L-1 and 100-300 mg.L-1, respectively. Similar results were obtained in conjunctival cell cultures by CK-119 and CK-122 at 3-10 mg.L-1 and 30-100 mg.L-1, respectively.

CONCLUSION

CK-119 and CK-122 are potent IL-1 blockers to inhibit cell growth of fibroblast-like corneal and conjunctival cells mainly through the inhibition of DNA and RNA syntheses but not protein synthesis.

摘要

目的

研究抑制成纤维细胞增殖的有效且无毒的药物。

方法

培养角膜和结膜成纤维样细胞，并用白细胞介素 -1（IL -1）阻断剂、二氢哒嗪并哒嗪类化合物CK -119和CK -122进行抑制。测定IL -1阻断剂孵育后细胞的生长以及DNA、RNA和蛋白质的合成情况。

结果

CK -119和CK -122在30 mg.L-1时抑制角膜成纤维细胞的生长。CK -119和CK -122显著抑制角膜成纤维细胞中的DNA和RNA合成，而蛋白质合成在30 - 100 mg.L-1和100 - 300 mg.L-1时分别未受影响或大多增强。在结膜细胞培养中，CK -119和CK -122分别在3 - 10 mg.L-1和30 - 100 mg.L-1时得到了类似结果。