（-）黄皮酰胺在体外对中枢胆碱能神经元的刺激作用。
Stimulation of central cholinergic neurons by (-)clausenamide in vitro.
作者信息
Duan W Z, Zhang J T
机构信息
Department of Pharmacology, Chinese Academy of Medical Sciences & Chinese Union Medical University, Beijing, China.
出版信息
Zhongguo Yao Li Xue Bao. 1998 Jul;19(4):332-6.
AIM
To study the neurotrophic effects of (-) and (+)clausenamide on frontal cortex neurons in culture.
METHODS
The activity of the choline acetyltransferase (ChAT) was determined by spectrophotometric method; protein content was assayed by Folin phenol method.
RESULTS
(-)Clausenamide increased the activity of ChAT and protein content in cultured neurons, as well as stimulated proliferation of neuronal cells, support survival and neurite outgrowth of neurons. The neurotrophic action of (-)clausenamide (0.001-10 mumol.L-1) was similar to that of nerve growth factor. The (+)clausenamide had no neurotrophic action, even at high concentrations (0.1-10 mumol.L-1), but neurons were damaged.
CONCLUSION
(-)Clausenamide stimulated central cholinergic neuron development.
目的
研究（-）和（+）黄皮酰胺对培养的额叶皮质神经元的神经营养作用。
方法
采用分光光度法测定胆碱乙酰转移酶（ChAT）的活性；采用福林酚法测定蛋白质含量。
结果
（-）黄皮酰胺可提高培养神经元中ChAT的活性和蛋白质含量，并刺激神经元细胞增殖，支持神经元存活和轴突生长。（-）黄皮酰胺（0.001 - 10 μmol·L-1）的神经营养作用与神经生长因子相似。即使在高浓度（0.1 - 10 μmol·L-1）下，（+）黄皮酰胺也没有神经营养作用，反而会损伤神经元。
结论
（-）黄皮酰胺可促进中枢胆碱能神经元发育。
Zotero 插件